Rabbit anti-Human IgG - Names and Identifiers
Rabbit anti-Human IgG - Physico-chemical Properties
Rabbit anti-Human IgG - Introduction
Rabbit anti-Human IgG is an antibody produced by rabbits that specifically recognizes and interacts with human IgG. It is widely used in many biological and immunological studies.
Its main properties are as follows:
1. Specificity: Rabbit anti-human IgG only reacts specifically with human IgG, and has no or little binding with immunoglobulins of other human immunoglobulin subclasses and species.
2. High purity: Rabbit serum is purified and purified to ensure its pure quality.
3. Stability: Rabbit anti-human IgG usually has good stability and can be stored and used under different experimental conditions.
Rabbit anti-human IgG has a wide range of applications in laboratory research and clinical diagnosis:
1. Immunodetection: Rabbit anti-human IgG is commonly used in the secondary antibody of immunoassay to detect the level of human IgG, such as enzyme-linked immunosorbent assay (ELISA), Western blot (Western blotting), etc.
2. Immunohistochemistry: Rabbit anti-human IgG can be used to detect the distribution and expression of human IgG in tissues.
3. Immunofluorescence: Rabbit anti-human IgG can be coupled with fluorescent dyes for specific staining of human IgG in cells or tissues.
The preparation of rabbit anti-human IgG usually includes the following steps:
1. Rabbit immunization: inject human IgG into rabbits to stimulate immune response and make rabbits produce antibodies against human IgG.
2. Serum preparation: Serum was collected from immunized rabbits, which contained immune antibodies.
3. Purification and purification: Rabbit anti-human IgG was isolated by serum purification and purification techniques.
When using Rabbit Anti-Human IgG, note the following safety information:
1. Rabbit anti-human IgG may cause allergic reactions and should be operated carefully to avoid contact with mucous membranes and skin.
2. It is recommended to wear personal protective equipment, such as lab clothes, gloves and goggles.
3. When storing and handling rabbit anti-human IgG, follow the instructions provided by the manufacturer to prevent contamination and degradation.
In summary, rabbit anti-human IgG is an antibody with high specificity and stability, which is widely used in immunological research and clinical diagnosis. Pay attention to safety measures during use to ensure the safety of experiments and operations.
Last Update:2024-04-09 19:06:00